[et_pb_section bb_built=”1″ _builder_version=”3.0.64″][et_pb_row][et_pb_column type=”4_4″][et_pb_text admin_label=”AUTHORS” _builder_version=”3.0.64″ background_layout=”light” text_orientation=”left” border_style=”solid” text_line_height=”1em”]

Lingling Yu, Jing Li, Ming Xiao

[/et_pb_text][et_pb_text admin_label=”INSTITUTIONS” _builder_version=”3.0.64″ background_layout=”light” text_orientation=”left” border_style=”solid” text_font_size=”11″]

Department of Dermatology of Shanghai Eighth People’s Hospital, Shanghai 200235, China

[/et_pb_text][et_pb_text admin_label=”SUMMARY” _builder_version=”3.0.64″ background_layout=”light” text_orientation=”left” border_style=”solid”]

SUMMARY

 

Purpose: Melanoma is one of the lethal types of skin malignancies and is responsible for significant morbidity and mortality across the globe. In this study the anticancer effects of picrocrocin on the human SK-MEL-2 malignant melanoma cell line were evaluated along with its mode of action.

Methods: The effect of picrocrocin was evaluated on SK-MEL-2 cells by MTT assay. Apoptosis was determined by DAPI and annexin V/PI staining. The effects on reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and cell cycle analysis were performed by flow cytometry. Expression of the proteins (JAK/STAT5) was estimated by western blotting.

Results: Picrocrocin exerted growth inhibitory effects on the SK-MEL-2 melanoma cells. The IC₅₀ of picrocrocin against the SK-MEL-2 cells was 20 µM at 24-h incubation. The antiproliferative activity of picrocrocin was due to apoptosis and cell cycle arrest. In addition, picrocrocin enhanced the ROS levels and decreased the MMP levels of SK-MEL-2 cells. Finally, picrocrocin inhibited the JAK/STAT5 signalling pathway in the SK-MEL-2 melanoma cells.

Conclusions: Taken together, these results indicate that picrocrocin can prove to be an important molecule in the search of more efficient therapies for melanoma.

Key words: anticancer, apoptosis, JAK, melanoma, picrocrocin

JBUON 2018; 23(4): 1163-1168 ISSN: 1107-0625, online ISSN: 2241-6293

[/et_pb_text][/et_pb_column][/et_pb_row][/et_pb_section][et_pb_section bb_built=”1″ _builder_version=”3.0.64″][et_pb_row _builder_version=”3.0.64″ background_position=”top_left” background_repeat=”repeat” background_size=”initial” background_position_1=”top_left” background_repeat_1=”no-repeat” background_position_2=”top_left” background_repeat_2=”no-repeat” background_position_3=”top_left” background_repeat_3=”no-repeat”][et_pb_column type=”1_3″][et_pb_button admin_label=”PDF” _builder_version=”3.0.64″ button_text=”Download PDF” url_new_window=”on” button_alignment=”left” background_layout=”light” custom_button=”on” button_letter_spacing=”0″ button_icon_placement=”right” button_letter_spacing_hover=”0″ button_text_color_hover=”#161616″ button_bg_color_hover=”#ffffff” button_url=”https://www.jbuon.com/pdfs/23-4/” /][/et_pb_column][et_pb_column type=”1_3″][et_pb_button admin_label=”PUBMED” _builder_version=”3.0.64″ button_text=”PubMed Abstract” url_new_window=”on” button_alignment=”center” background_layout=”light” custom_button=”on” button_letter_spacing=”0″ button_icon_placement=”right” button_letter_spacing_hover=”0″ button_text_color_hover=”#161616″ button_bg_color_hover=”#ffffff” /][/et_pb_column][et_pb_column type=”1_3″][et_pb_button admin_label=”Issue Contents” _builder_version=”3.0.64″ button_text=”Issue Contents” url_new_window=”off” button_alignment=”right” background_layout=”light” custom_button=”on” button_letter_spacing=”0″ button_icon_placement=”right” button_letter_spacing_hover=”0″ button_text_color_hover=”#000000″ button_bg_color_hover=”#ffffff” button_url=”https://jbuon.com/23-4″ /][/et_pb_column][/et_pb_row][et_pb_row][et_pb_column type=”4_4″][et_pb_text admin_label=”Creative Commons” _builder_version=”3.0.64″ background_layout=”light” text_orientation=”left” border_style=”solid” text_line_height=”1em” text_font_size=”12″]

 This work by JBUON is licensed under a Creative Commons Attribution 4.0 International License.

[/et_pb_text][/et_pb_column][/et_pb_row][/et_pb_section]