Ziqi Cheng, Yapei Lu, Yuan Fang
Department of Gynecology, the First Hospital of Hangzhou Fuyang, Hangzhou, China
Summary
Purpose: This study aimed to investigate the influence of Fucoidan on changing ovarian cancer cell functions through the miR-20a-5p/PKD2 axis.
Methods: Ovarian cancer cell lines SKOV3 and CAOV3 were treated with DMSO (negative control), 20 ng/ml Fucoidan, 50 ng/ml Fucoidan, 100 ng/ml Fucoidan, or 200 ng/ml Fucoidan for 24 hours, followed by fresh medium replacement and cell culture for another 48 hours. The proliferation inhibition rate was assessed using the CCK-8 assay. Proliferative and migratory rates in SKOV3 and CAOV3 cells induced with 100 ng/ml Fucoidan for 24 hours were determined by CCK-8, EdU, and Transwell assays. The relative levels of miR-20a-5p and PKD2 in Fucoidan-induced cells were detected. The regulatory effects of miR-20a-5p on phenotypes of Fucoidan-induced ovarian cancer cells were examined. The binding interaction between miR-20a-5p and PKD2 was assessed using dual-luciferase reporter assay and rescue experiments.
Results: Fucoidan induction dose-dependently enhanced the proliferation inhibition rate in SKOV3 and CAOV3 cells and significantly inhibited proliferative and migratory functions in ovarian cancer cells. Fucoidan induction upregulated miR-20a-5p and downregulated PKD2 expression. The binding relationship between miR-20a-5p and PKD2 was confirmed. Overexpression of miR-20a-5p reduced proliferative and migratory functions in Fucoidan-induced ovarian cancer cells, which were reversed by overexpressed PKD2.
Conclusions: Fucoidan inhibits ovarian cancer cell proliferation and migration via mediating the miR-20a-5p/PKD2 axis.
Keywords: fucoidan, miR-20a-5p, PKD2, ovarian cancer, proliferation, migration.
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